The Novel Method in Bacteriuria Screening

抄録

Point of care testing, a rapid and easily accessible exam that provides a quick result to the patients, is an emerging technique in the medicare system. This technique facilitates better disease diagnosis, monitoring, and management. It enables more precise medical decisions, as the diseases can be diagnosed at a very early stage, leading to improved health outcomes for patients by allowing the immediate start of treatment. It also provides patients to monitor their disease control more efficiently. The finger sugar testing is an excellent example of these techniques. Diabetic patients can check their blood sugar at any time and anywhere and modify their anti-diabetic medication or insulin injection to avoid blood sugar over or under control. For the non-diabetic ones, or who are unaware of getting diabetes, they can check their fasting sugar at home and seek the doctor earlier if the rapid testing reported abnormal fasting sugar.

Urinary tract infection (UTI) is a common disease. The prevalence in women over 65years of age is approximately 20% and nearly 11% in the overall population. Meanwhile, UTI accounted for the second most of the cause of sepsis during hospitalization. The gold-standard diagnosis of urinary tract infection is made by clinical symptoms and positive results of urine culture. Because of the variety of clinical manifestations of urinary tract infection and the time-consuming urinary culture, the diagnosis of urinary tract infection is rather difficult. The current urinary dipstick was not reliable with low sensitivity in the diagnosis of UTI. Although empirical broad-spectrum antibiotics are effective in treating UTI, these uncertainties in determination lead to the overuse of antibiotics in clinical practice. Drug resistance pathogens are result from the overuse of antibiotics. Therefore, the more accurate and rapid test for UTI is urgent.

MTT+ Assay (3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromid) is a colorimetric assay for assessing cell metabolic activity. In other words, the method could quantify the living cell of the sample. Our Lab had applied this exam on the sperm mobility examination previously. In previous bacterial in vitro experiments, we had found the colorimetric result might be correlated to the concentration of bacteria. As a result, we plan to conduct an MTT assay for the urinary bacteria examination in vivo. With the rapid result of MTT assay, it could improve our current bacteriuria screen. We also plan to develop a Point-of- Care Testing device with MTT assay to exam the bacteriuria more efficiently.