Matrix-stiffness-regulated Migration and Proliferation of Tumor Spheroids

抄録

Malignant cancer cells motorize to the distal tissues by invasion and proliferation. These distal tissues have mechanical differences from the brain, 1~2 kPa, to the bone, 25~40 kPa. The coupling of matrix stiffness on cell proliferation remains indecisive to the durotaxis-driven motility. In this study, we exposed the A549 adenocarcinoma organoid on stiffness adjustment collagen construct to examine the influence of collective motility and cell proliferation. Centrifugation compressed collagen construct was developed for the physical adjustment of matrix stiffness. The collagen construct was stiffened from 2.9 kPa to 17.2 kPa. Over 7 days, the spheroids expanded larger on the uncompressed collagen construct than those on the compressed. However, the number of proliferated cells in spheroids showed no difference between the uncompressed and compressed collagen constructs. The preliminary results indicate that the matrix stiffness guides cells mainly on motility rather than proliferation.