医用電子と生体工学
Online ISSN : 2185-5498
Print ISSN : 0021-3292
ISSN-L : 0021-3292
パッチクランプ測定装置用磁場曝露デバイス
大保 真由美大高 好久北村 敏
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ジャーナル フリー

2001 年 39 巻 3 号 p. 197-202

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A device was developed that enables in situ optical and electrical observations of a single cell under exposure to a magnetic field. Electric current through a flat conductor sheet made a homogeneous magnetic field above the sheet. The sheet was made of brass, 30mm×30mm to 60mm×660mm and 0.3mm thick, with a hole 2mm in diameter in the center of the sheet for the light path of a microscope. An electric current of diverse waveforms was supplied by a function generator through an amplifier, generating a magnetic field of the same waveform up to 0.14mT. The sheet was laid concentrically on the stage of a microscope. The homogeneity of the field over a 40mm×40mm sheet was estimated to be 5% in the vertical and 0.1% in the horizontal directions for a 1mm shift of a cell. A sheet does not hinder movement of the patch-clamp attachments and leaves space for a pair of coils to compensate the geomagnetic field on the stage of the microscope. The electric noise caused by the field was so small that it could be canceled in the data collection process.
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© 日本生体医工学会
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