Microbes and Environments
Online ISSN : 1347-4405
Print ISSN : 1342-6311
ISSN-L : 1342-6311
Regular Papers
Population of Diazotrophic Endophytes in Stem Apoplast Solution of Sugarcane and Related Grass Species in Tanegashima, Japan
Constancio A. Asis, Jr.Katsuki AdachiAkira SugimotoKunihiro UjiharaYoshifumi TerajimaSeiji Fukuhara
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2003 Volume 18 Issue 3 Pages 133-137


We collected stem samples from sugarcane germplasm of wildtype (43 clones of Saccharum spontaneum and S. robustum), cultivated (34 clones of S. hybrid spp., S. sinense, and S. barberi), and related grass species (7 clones of Erianthus arundinaceus, Miscanthus floridulus, and Ripidium sp.) to survey the colonization and population of diazotrophic endophytes in apoplast solution. Using the acetylene reduction assay-most probable number (ARA-MPN) method, 11 clones of wildtype species, nine clones of cultivated species, and three clones of related grass species were colonized by diazotrophic endophytes. The population density of diazotrophic endophytes in all ARA-positive samples ranged from 102 to 108 cells mL-1 apoplast solution and differed among clones and species. The results indicate a differential colonization and population of diazotrophic endophytes in stem apoplast solution.

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© Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology
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