Identification of Aspergillus nomius in Bees Visiting Brazil Nut Flowers

Fernanda Pelisson Massi; Rafael Elias Silva Penha; Marcelo Casimiro Cavalcante; Helena Paula Viaro; Josué José da Silva; Larissa de Souza Ferranti; Maria Helena Pelegrinelli Fungaro

doi:10.1264/jsme2.ME14146

Short Communications

Identification of Aspergillus nomius in Bees Visiting Brazil Nut Flowers

Fernanda Pelisson Massi, Rafael Elias Silva Penha, Marcelo Casimiro Cavalcante, Helena Paula Viaro, Josué José da Silva, Larissa de Souza Ferranti, Maria Helena Pelegrinelli Fungaro

著者情報

キーワード: aflatoxin, Aspergillus section Flavi, molecular detection, pollinators of Bertholletia excelsa

ジャーナルフリー HTML

2015 年 30 巻 3 号 p. 273-275

DOI https://doi.org/10.1264/jsme2.ME14146

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詳細

抄録

We designed a primer pair (BtubNomF/BtubNomR) specifically for amplifying Aspergillus nomius DNA. In vitro assays confirmed BtubNomF/BtubNomR specificity, corroborating its usefulness in detecting and identifying A. nomius. We then investigated the occurrence of A. nomius in floral visitors of Bertholletia excelsa trees by means of PCR, and A. nomius was detected in the following bees: Xylocopa frontalis, Bombus transversalis, Centris denudans, C. ferruginea, and Epicharis flava. The presence of A. nomius in bees visiting Brazil nuts opens up new avenues for obtaining novel insights into the process whereby Brazil nuts are contaminated by aflatoxin-producing fungi.

責任著者(Corresponding author)

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