Identification of Aspergillus nomius in Bees Visiting Brazil Nut Flowers

Fernanda Pelisson Massi; Rafael Elias Silva Penha; Marcelo Casimiro Cavalcante; Helena Paula Viaro; Josué José da Silva; Larissa de Souza Ferranti; Maria Helena Pelegrinelli Fungaro

doi:10.1264/jsme2.ME14146

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Identification of Aspergillus nomius in Bees Visiting Brazil Nut Flowers

Fernanda Pelisson Massi, Rafael Elias Silva Penha, Marcelo Casimiro Cavalcante, Helena Paula Viaro, Josué José da Silva, Larissa de Souza Ferranti, Maria Helena Pelegrinelli Fungaro

著者情報

キーワード: aflatoxin, Aspergillus section Flavi, molecular detection, pollinators of Bertholletia excelsa

ジャーナルフリー早期公開

論文ID: ME14146

DOI https://doi.org/10.1264/jsme2.ME14146

この記事には本公開記事があります。

The final version of this article is now available: Vol. 30 (2015), No. 3 pp. 273-275

詳細

抄録

We designed a primer pair (BtubNomF/BtubNomR) specifically for amplifying Aspergillus nomius DNA. In vitro assays confirmed BtubNomF/BtubNomR specificity, corroborating its usefulness in detecting and identifying A. nomius. We then investigated the occurrence of A. nomius in floral visitors of Bertholletia excelsa trees by means of PCR, and A.nomius was detected in the following bees: Xylocopa frontalis, Bombus transversalis, Centris denudans, C. ferruginea, and Epicharis flava. The presence of A. nomius in bees visiting Brazil nuts opens up new avenues for obtaining novel insights into the process whereby Brazil nuts are contaminated by aflatoxin-producing fungi.

責任著者(Corresponding author)

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