Microbes and Environments
Online ISSN : 1347-4405
Print ISSN : 1342-6311
ISSN-L : 1342-6311
Short Communication
Kinetics of Nitrous Oxide (N2O)-reducing Activity of Bradyrhizobium ottawaense by an Automated Analysis
Manabu Itakura , Kiwamu Minamisawa
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2026 年 41 巻 1 号 論文ID: ME25070

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The biological reduction of N2O, a potent greenhouse gas, is crucial for environmental sustainability. We developed an automated system for continuous N2O monitoring in the gas phase of a flask containing an anaerobic bradyrhizobial culture, and then exami­ned the kinetic parameters of bacterial N2O reduction. The maximum reaction rate (Vmax) was approximately 61-fold higher for Bradyrhizobium ottawaense SG09 (1,471‍ ‍nmol h–1 109 cells–1) than for B. diazoefficiens USDA110 (24‍ ‍nmol h–1 109 cells–1). Our kinetics anal­ysis confirmed that SG09 maintained higher N2O-reducing activity than USDA110 even at the atmospheric concentration of N2O (0.34 ppm).

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© 2026 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles.

This article is licensed under a Creative Commons [Attribution 4.0 International] license.
https://creativecommons.org/licenses/by/4.0/
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