Secondary Structural Analyses of ITS1 in Paramecium

Ryo Hoshina

doi:10.1264/jsme2.ME10129

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Secondary Structural Analyses of ITS1 in Paramecium

Ryo Hoshina

著者情報

キーワード: internal transcribed spacer 1 (ITS1), Paramecium, phylogeny, secondary structure

ジャーナルフリー早期公開

論文ID: ME10129

DOI https://doi.org/10.1264/jsme2.ME10129

この記事には本公開記事があります。

The final version of this article is now available: Vol. 25 (2010) , No. 4 p.313

詳細

抄録

The nuclear ribosomal RNA gene operon is interrupted by internal transcribed spacer (ITS) 1 and ITS2. Although the secondary structure of ITS2 has been widely investigated, less is known about ITS1 and its structure. In this study, the secondary structure of ITS1 sequences for Paramecium and other ciliates was predicted. Each Paramecium ITS1 forms an open loop with three helices, A through C. Helix B was highly conserved among Paramecium, and similar helices were found in other ciliates. A phylogenetic analysis using the ITS1 sequences showed high-resolution, implying that ITS1 is a good tool for species-level analyses.

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