抄録
In this study, we observe intracellular calcium signaling response to stretch in a single osteoblastic cell with video rate temporal resolution. Our originally developed cell stretching microdevice enables in situ observation of stretched cell without excessive motion artifact such as focus drift. Minor effect of motion artifact was corrected by using fluorescent ratiometry method with calcium indicators Fluo 8H and Fura Red. We succeed to detect intracellular calcium signaling response to stretch with video rate temporal resolution.
