Abstract
In this study, we developed the intuitive remote controlled manipulator system using touch panel display and mouse, and we examined the possibility of the application to the extraction work of the shoot apical meristem of the plant. The shoot apical meristem does not have the infection of the virus in the plant body. Therefore we can get a Virus-free seedling having high production efficiency by culturing this part. This technique is called "mericlone" and is known widely in the agriculture world, but this is hard labor because of precision work with microscope. This system we have developed not only reduce the burden on worker but also provide high precision controlling and space saving. In this study, with LAMP method (loop-mediated isothermal amplification method), we have confirmed that there was no PVX (Potato virus X) in the potato meristem with primordia using our developed manipulator system The newly designed primer set can discriminate the result.