Abstract
Techniques of DNA manipulation are important for molecular biology. In this study, we propose a novel high-throughput DNA fiber assembling method to semi-control DNA positioning. We fabricated SU-8 microbridges using the Single Mask Inclined Photo-Lithography Expose (SIMPLE) process, and stretched the human chromosome on those microbridges by centrifugal force. Then DNA fibers can be stretched separately on microbridges, which are called a Genetic Extended-fiber Network (GEN). By performing Fluorescent In Situ Hybridization (FISH) analysis, the validity of GEN using the microbridges for DNA analysis was confirmed.
