血管網付与による立体心筋組織構築のための新規灌流培養法の検討

抄録

Thick three-dimensional (3-D) cardiac tissues have a great future potential in regenerative medicine for severe heart disease. Our laboratory has developed a fabrication method of cardiac tissues by stacking cell sheets. However, there is a limitation for stacking of cell sheets because of the cell necrosis due to lack of oxygen or nutrition. Therefore, for creating a thick tissue, it is necessary to supply oxygen and nutrients by sufficient vascular networks into the stacked cell sheet tissue and perfusion culture. In this study, we developed a new technology for vascularization with a tissue perfusion bioreactor system in vitro and attempted to create a cardiac tissues.