DNA１分子サイズ測定のための微小流路内の気液界面の移動を用いた分子の伸長・固定～分子伸長率の気液界面の移動速度依存性～

抄録

In this study, we stretched and immobilized a 48.5 kbp-DNA molecule using a pressure flow and a gas-liquid interface movement in a microchannel. A chip device with a straight microchannel was fabricated by transfering Si mold created by a photolithography to PDMS. The depth and width of the microchannel were 2 μm and 100 μm, respectively. We controlled the velocities of the pressure flow and the gas-liquid interface by controlling applied pressures into the microchannel in order to investigate the dependence of stretched rate of the DNA molecule on the velocities. We measured the sizes of stretched and immobilized DNA molecules in the microchannel at each applied pressure by analysing the fluorescent images obtained using a fluorescence microscope. We indicated that the increase of the applied pressure improved the stretched rate of DNAs and achieved the stretched rate of 70 % under the condition of 1150 μm / s (600 mbar).