抄録
Nanopore sequencer is expected as a core technology for realizing medical treatment based on genome information. In order to improve the analysis efficiency, it is necessary to be able to analyze a large molecular size genomic DNA (large molecular weight DNA) with one molecule. Large molecular weight DNA has a random coil structure in a stable state. Therefore, it is indispensable to establish the technology to extend the random coil-shaped DNA molecule linearly in order to realize the analysis with the nanopore sequencer. In this study, we experimentally verified the effect of migration conditions on the elongation rate of DNA molecules that elongate during electrophoresis in a nanoslit. The results showed that DNA elongation was significantly affected not only by the geometric conditions of the flow channel, but also by the electric field distribution (position within the nanoslit) and the migration speed.
