IIP情報・知能・精密機器部門講演会講演論文集
Online ISSN : 2424-3140
IIP2020 情報・知能・精密機器部門講演会
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ナノスリット流路を電気泳動する大分子量DNAの分子伸長とその電圧依存性
*永田 春香伊藤 伸太郎東 直輝福澤 健二張 賀東
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会議録・要旨集 認証あり

p. 2C05-

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Nanopore sequencer is expected as a core technology for realizing medical treatment based on genome information. In order to improve the analysis efficiency, it is necessary to be able to analyze a large molecular size genomic DNA (large molecular weight DNA) with one molecule. Large molecular weight DNA has a random coil structure in a stable state. Therefore, it is indispensable to establish the technology to extend the random coil-shaped DNA molecule linearly in order to realize the analysis with the nanopore sequencer. In this study, we experimentally verified the effect of migration conditions on the elongation rate of DNA molecules that elongate during electrophoresis in a nanoslit. The results showed that DNA elongation was significantly affected not only by the geometric conditions of the flow channel, but also by the electric field distribution (position within the nanoslit) and the migration speed.

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