A lot of handicapped people feel endangered when they are transported by a vehicle sitting on a wheelchair. However, there is no safety standards on function or fixing of wheelchair with a car and a sitting of a human on a wheelchair. This paper deal with a human body response on-vehicle wheelchair during vehicle travel. A wheelchair is fixed with the wagon specially designed for wheelchair transporting with four-point fixing method and Acceleration, electro level muscles and deflection angles of some joints of human body are measured for various driving conditions of the vehicle. Data are compared with each other and the problem and the effectiveness of these data are discussed.