抄録
Knowledge on cell disruption by physical stresses is applicable to material introduction and sterilization, etc. In this study, the influences of various physical stresses on E. coli cells were evaluated. Cell viability decreased according to strength and loading time of physical stresses examined, i.e. shear stress, ultrasonic and pressure change. The quality of cell disruption, observed by scanning electron microscopy, differed between these physical stresses. The quantity of water-soluble protein spilled out from cells after loading of stresses increased with concomitant decreasing in cell viability. These results suggest that the disruption of E. coli cells by loading of stresses accompanied with outflow of cytoplasm. Insertion of relaxation time elongated cell viability. These results are discussed in application point of view.
