抄録
During mitosis, sister chromatids are separated by several forces including the pulling force toward two sister centrosomes via microtubules. This pulling force was typically evaluated with laser micro-surgery, which disrupts microtubule structures irreversibly. This irreversible disruption disables long term observation during whole mitosis. In this study, the pulling force was intended to measure with traction force microscopy, which evaluates cellular traction force without any disruptions. Especially, in this paper, existence of two chemicals, fetal bovine serum (FBS) and phenol-red, which are indispensable for long term observation, are tested whether they disturb fluorescence imaging which is necessary for traction force microscopy.
