A series hybrid electrical vehicle with a bio fuel diesel engine generator, named as bio EV, is proposed in this paper. The diesel engine rotates an electric generator using straight bio fuels such as sun flower oil. The hybrid electric vehicle is able to run using the generated electricity. The electric generator is also able to charge the electricity to batteries. It is expected to realize a long distance running electrical vehicle, and also a carbon neutral system. This paper will present the experimental results of the fuel effects.