抄録
It has been reported that glycans play pivotal roles in biomechanical responses of cells. To reveal the roles of glycans in mechanical response of endothelial cells, we observed morphological changes of a murine vascular endothelial cell line F-2 treated with a drug DG, which modifies protein glycosylation. When a confluent cell monolayer was treated with DG in static culture, it formed mesh-like structure in 48 hours. To reveal the reason for the mesh-like structure formation, we measured adhesion strength between the cells and that between the cell and the substrate with laboratory-made testers and found that the mesh-like structure formation was accompanied by an increase in the intercellular adhesion strength and a decrease in the cell-substrate adhesion strength. These changes may facilitate local aggregation of cells in a cell monolayer and thus cause the mesh-like structure.
