This paper reports on measurement of ground reaction forces (GRFs) of ant legs during running on level ground, vertical wall and ceiling. Micro force plate array is designed and fabricated to measure anterior and vertical directional forces. Thus, GRF of all legs in the various ground angles can be detected simultaneously. The size and force resolution are 2000 μm × 1000 μm × 20 μm and 1 μN, respectively. The measurement results show that ants use different GRF distribution in three types of terrestrial locomotion. In every types of locomotion, the ant was affected by the gravity reaction force, which was totally equal to the body weight. The gravity reaction force was approximately equal to each leg during running on level ground and ceiling. The gravity reaction force during ceiling was thought to be generated by the adhesion force of sub-micron scale hairs on the tip of the legs. The measured GRF distribution clarified that ants use the different strategies of running mechanism depending on the angle of the ground against the horizon plane.