抄録
A soft contact-lens (SCL) glucose sensor for non-invasive monitoring of tear glucose concentration on an eye site was fabricated and demonstrated. Wearing the SCL sensor on the eye enabled in situ monitoring of glucose content of tears. The SCL sensor consists of an enzyme membrane and an SCL flexible device with Pt & Ag/AgCl electrodes, which were fabricated by micro-electro-mechanical systems (MEMS) techniques. The electrodes at the sensing region were coated with glucose oxidase (GOD) with biocompatible polymer. The SCL sensor showed a good relationship between the output current and glucose concentration in the range of 0.03 - 5.0 mmol/l (covering the tear glucose level in normal and diabetic patients), with the correlation coefficient of 0.999. In order to evaluate the tear static and dynamic states, the SCL sensor was applied to a rabbit for the ocular biomonitoring. The SCL sensor allowed real-time monitoring of the glucose concentration changes in tears induced by ocular glucose instillations. The average tear turnover rate was estimated to be 29.6 %/min.
