基質材料種・性状が破骨細胞代謝・溶解挙動に及ぼす影響の評価

抄録

In this study, osteoclasts are cultured on the substrates with the different molecular weight, elastic modulus and material type, in order to investigate the influence of the substrate material type and properties on the osteoclast metabolic and desorption behavior. Here, PCL and chitosan were used as materials for substrates to culture osteoclasts. The PCL substrates ware prepared by changing the cooling rate to change the degree of crystallinity and subsequent elastic modulus. The Chitosan substrates with different elastic modulus were prepared by changing the degree of crosslinking. Osteoclasts were then cultured on the prepared substrate for 48 hours. The substrate mechanical properties were evaluated by tensile tests. As a result, neither the PCL substrate nor the chitosan substrate showed the marked difference in the mechanical properties of the substrate due to the metabolic behavior of osteoclasts and hydrolysis. It is considered that the incubation period of osteoclasts as an important factor for the interaction between the metabolic behavior of osteoclasts and the mechanical properties of the substrate was short.