プラズマ表面改質法をもちいた細胞接着性に関する研究

抄録

In Tissue Engineering developed by Langer and Vacanti, it is known that lost tissues and organs can be regenerated artificially. Tissue engineering requires three elements, i.e., cell, growth factor, and biological material for cell scaffold, and these elements interact with each other. Thereby, the original regeneration ability of organisms can be enhanced. The surface properties have been considered to affect to material and cell interaction because a cell contacts directly with the surface of a biomaterial. Therefore, in this study, the change of the surface state of the material by plasma surface treatment and the influence on the cell adhesiveness were investigated. Since PET has been employed as cell scaffold, its adhesiveness to a PET plate in vortex flow was investigated. Although the surface of PET temporarily became hydrophilic by the plasma surface treatment, the contact angle changed with time and reached to the same value as that of the control material after about 9 days. The surface chemistry by XPS measurement was found that the surface was oxidized. In this study, the decrease in the contact angle and the increase in the surface oxygen content by the plasma treatment were found to affect to the cell adhesiveness to PET plate.