細胞動態の長期オンライン計測に向けた脱水素酵素型グルコースセンサの開発

抄録

In medical and drug discovery studies, it is necessary to investigate efficacy of drug candidates under development. Conventionally, cell-based assays using human cells are widely carried out as an alternative to animal testing for this purpose in vitro. However, it is difficult to evaluate the effectiveness over time of drugs using conventional methods which are not capable of measuring cell kinetics. Currently, microfluidic devices integrated with a glucose sensor have been proposed for measurement of cell kinetics in the research field of μTAS (Micro Total Analysis Systems). Because cells consume glucose as an energy source, glucose consumption is an important indicator related to their activities. We aim to develop a microfluidic device integrated with a glucose dehydrogenase (GDH) based glucose sensor with a dialysis membrane for long-term online measurement of cell activities. In this study, we evaluated function of a GDH-based glucose sensor with a dialysis membrane that we developed for long-term measurement. The results showed that a dialysis membrane prevent the blood serum protein adheres to the sensing membrane, suggesting that it is possible to measure cell kinetics long-term on-line on chips.