３次元骨細胞組織モデルを用いた細胞バイオメカニクス

抄録

Osteocytes modulate osteoclasts for bone resorption and osteoblasts for bone formation during the bone remodeling process. In this study, we elucidated the effect of 3D culture on the osteocyte differentiation. We fabricated self-organized spheroids reconstructed by mouse pre-osteoblast cells. The cells in 3D tissue-engineered model rendered the osteocyte-likeness within 2 days. We also showed that the cell condensed condition achieved from the 3D culture is essential for up-regulations of osteocyte gene expressions. Moreover, compared to the conventional monolayer model, the 3D culture model exhibited a different response to external stimuli such as hypoxia. In this study, we broadened a new insight into the osteocyte differentiation capability of pre-osteoblast cells using the 3D culture model.