G0200-1-5 傾斜遠心顕微鏡を用いたガラス平板上におけるHL60の微視的挙動に関する基礎的研究(バイオエンジニアリング部門一般講演(1),社会変革を技術で廻す機械工学)

抄録

The authors have investigated the adhesion and friction characteristics of the neutrophils on a flat glass plate using the inclined centrifuge microscope. In their studies, however, precise behavior of the neutrophils on the plate has not been observed. In this study, microbeads were attached on the HL60 cells, a model cell of neutrophils, via an antibody as marker particles to observe if the cells were rolling or sliding on the plate. Some cells were observed to roll on the glass plate and showed the stick-slip motion. Influence of stimulation of the HL60 cells by the antibody on their adhesivity to the plate was also investigated with respect to the adhesion ratio. It was shown that the adhesion ratio of the stimulated cells did not change with coating of the plate with the MPC polymer which prevents the cells adhesion, while that of the not-stimulated cells decreased drastically.