P-MCH-03 LOCALIZED SUBSTANCE DELIVERY TO SINGLE CELL BY THREE DIMENTIONAL MICROFLUIDIC DEVICE(Micro/Nanomechatronics,Technical Program of Poster Session)

Kyohei TERAO; Murat GEL; Hiroki KOTAKE; Atsuhito OKONOGI; Teru OKITSU; Takaaki SUZUKI; Masao WASHIZU; Hidetoshi KOTERA

doi:10.1299/jsmemipe.2009.385

2009

Session ID : P-MCH-03

DOI https://doi.org/10.1299/jsmemipe.2009.385

Conference information

P-MCH-03 LOCALIZED SUBSTANCE DELIVERY TO SINGLE CELL BY THREE DIMENTIONAL MICROFLUIDIC DEVICE(Micro/Nanomechatronics,Technical Program of Poster Session)

Kyohei TERAO, Murat GEL, Hiroki KOTAKE, Atsuhito OKONOGI, Teru OKITSU, Takaaki SUZUKI, Masao WASHIZU, Hidetoshi KOTERA

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CONFERENCE PROCEEDINGS FREE ACCESS

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Abstract

We propose a three dimensional micro-fluidic device for the analysis of intracellular response to localized chemical stimulation. The device has microchannels and an aperture in their side wall, where single cell is immobilized and subjected to chemical stimuli. The device was fabricated using PDMS molding of SU-8 structure and self-forming polymer bridge. Delivery of fluorescent dye, calcein, was demonstrated here.

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