Changes in surface roughness of SUS316NG during cyclic variable loadings were investigated. Strain controlled two-step fatigue tests were conducted under two different strain ranges. In the tests, strain range was switched from Δε_H=4% to Δε_L=2% or from Δε_L=2% to Δε_H=4% at the usage factor UF=0.2. The specimen's surface was observed by a laser scanning microscope to clarify the surface profile and surface roughness R_a. As a result, R_a increased with UF during the first and second steps in the two-step tests. The increasing rate of R_a at the second step became smaller after strain range was changed from 4% to 2%, whereas it became larger after strain range was changed from 2% to 4%. The increasing tendencies of Ra at each step were similar to those in fatigue test under constant strain ranges Δε=4% and 2%, respectively. The results suggest that changes in surface roughness under two-step variable loadings can be described by superimposing R_a values in order of loading, which were obtained under constant strain range tests. Surface observation revealed that the height change in surface profile during each loading step varied depending on the strain range. This shows that the history of strain range affected the changes in surface profile, and it is the reason that the increasing rate of R_a changed in the two-step tests.