Bone is often regarded as a composite material consisting of mineral particles and organic matrix (mostly Type I collagen) on a microscopic scale. The mechanical properties of bone tissues at a macroscopic scale depend on the structural organization and properties of constituents in the microscopic scale. However, both mechanical behaviors of mineral and collagen phases are not clear yet. In this study, both microscopic strain of collagen and mineral phases in cortical bone by using small and wide angle X-ray diffraction, respectively. The collagen matrix strain allowed tensile deformation as compared with mineral phase. This study could suggest that macroscopic mechanical properties of bone tissue depend on the degree of deformation of mineral and collagen matrix.