抄録
We are aiming to control the osteoclastic metabolic behavior by the material design of substrates, in order to accelerate the absorption of scaffolds for bone regeneration. We hypothesized that the metabolic behavior of osteoclasts would activate with increasing elastic modulus of substrates. The purpose of this study is to demonstrate this hypothesis by comparing the metabolic behavior of osteoclasts on substrates with different elastic modulus. Here, hematopoietic stem cells were cultured and differentiated into osteoclasts on PCL substrates. The elastic modulus of PCL substrates was controlled as 0.295 GPa, 0.406 GPa and 0.657 GPa by changing cooling rate as rapid cooling, slow cooling and cooling in furnace, respectively. The metabolic behavior of osteoclasts was observed by fluorescence imaging of cathepsin K. As results, activation ratio of osteoclasts was 36.5 %, 57.4 % and 62.5 % on rapidly cooled, slowly cooled and furnace-cooled PCL substrate, respectively. Thus, it can be verified that the increase in substrate elastic modulus induces the activation of osteoclastic metabolic behavior.
