皮質骨有機・無機ラマンシフトの負荷応答と無機体積分率の影響

抄録

Bone tissue with hierarchical structure is regarded as complex structure consisting of organic collagen and inorganic hydroxyapatite (HAp). Using the component analysis method called Raman spectroscopy, the change in Raman shift was measured while applying load to bone tissue, and the relationship between stress and Raman shift was investigated. In addition, the Raman shifts were measured by statistical process based on the Raman images. The specimens with the sizes of 10 × 2 × 0.5 mm were cut out from 23 m. o. bovine femoral diaphysis, where the longer edges of specimens are aligned to the parallel to the femoral axis. Decalcified specimens with 10% formic acid (CH₂ O₂) for 4 hours were prepared. Strain gauges were attached to bovine cortical bone specimens, and Raman spectrum was measured while applying tensile stress. The Raman shifts were calculated by averaging obtained peak shifts at all measurements point. As the results, the mechanical behaviors of collagen matrix in demineralized bone were found by Raman spectroscopy.