Abstract
We have developed a micro-fabricated chip to separate DNA molecules by their molecular sizes based on the principle of the size exclusion chromatography (SEC). However, there was a problem of resolution lowering due to the spreading of DNA molecules in the direction of electrophoresis when they entered into the area where the micro-structures for molecular sieving were formed. In this study, we fabricated DNA separation tip that has nano-slit structure for online concentration of DNA samples and experimentally verified the effectiveness of the preconcentration. Experimental results showed that the preconcentration was effective for the improvement of detection sensitivity and resolution of size separation.
