The Proceedings of the Symposium on Micro-Nano Science and Technology
Online ISSN : 2432-9495
2013.5
Session ID : 6AM2-D-3
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6AM2-D-3 Preconcentration of macromolecules for efficient on-chip cell electroporation
Yuko JuriRyuji YokokawaHidetoshi KoteraHirofumi Shintaku
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CONFERENCE PROCEEDINGS FREE ACCESS

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Abstract
In this study, we propose an efficient on-chip cell electroporation that utilizes preconcentration of plasmids by isotachophoresis. The microfluidic device is a simple straight microchannel with a constriction that strengthens the electric field for the electroporation. We leverage the isotachophoresis to preconcentrate the plasmids in the constriction for efficient delivery of the molecules into living cells. We here demonstrate the feedback position control of the preconcentrated layer based on pressure driven flow and electrical current. The designed feedback system successfully shows the stabilization of the position of the preconcentrated layer in the constriction.
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© 2013 The Japan Society of Mechanical Engineers
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