Abstract
A patterining technique of single cells is required for studying cell-cell communication. The objective of this research is to develop a versatile cell manipulation system capable of patterning of single cells in high precision and super parallel format. We develop cell manipulation technique by an electroosmotic (EO) flow with a microprobe-array and micropipette. Application of DC voltage (5 V) generated an EO flow through the hollow channels of the probe-array and pipette. Cells moved toward tips of the micro-probe array or the pipette by EO flow. The cells were tracked and analyzed. Cell damage was characterized under optical observation. A cell swelling and loss of the fluorescence was observed 180 s after the cell capture.
