原子間力顕微鏡を用いた細胞外小胞に対するイオン枯渇領域の斥力影響の評価手法の開発

抄録

Much attention has been paid to the exosome which is a small vesicle working as a tool for cell-cell communication in our body. To clarify unexplained phenomena related with the exosome, a smart technique of exosome handling has been strongly demanded to treat such a delicate substance. For resolution of this issue, we used ion-depletion zone for treating bio-substances including exosome without physical loads. We fabricated a prototype device, and demonstrated on the device to form an ion-depletion zone. The repulsive force of ion-depletion zone was visualized by fluorescent imaging of exosomes pushed out from the ion-depletion zone, and it was evaluated by measuring the zone dimension. After the demonstration, the shape and amount of the exosomes were observed by using an atomic force microscope to estimate the effects of the technique on the sample.