抄録
We studied the properties of giant unilamellar liposomes (GUVs) containing hydrogel as a dynamic microcontainer to construct digital microfluidic system that works in the aqueous environment. Here we especially focus on the release of encapsulated molecules. For conventional liposomes, the poration of lipid membrane is led by applying DC pulses. However, the formed pores are rapidly resealed due to the lipid diffusion in the membrane, resulting in the leakage of only a portion of enclosed molecules. We found that hydrogel formed within GUVs stabilizes the transiently formed pores. As a result, almost all the enclosed molecules can be released to the outer environment.
