Abstract
Laser manipulation, which is well known as optical tweezers, is one of the most powerful techniques for manipulating micron-sized objects under an optical microscope. Three-dimensional (3D) manipulation of arbitrarily shaped objects under an optical microscope is of widespread importance. We have developed two PC controlled 3D laser manipulation systems with excellent user-interfaces and image processing functions. In this paper we show the results of several demonstrations using these systems; namely the 3D manipulation of micron-sized objects with non-spherical shape (for example, whisker, diatom and so on).