The Proceedings of JSME annual Conference on Robotics and Mechatronics (Robomec)
Online ISSN : 2424-3124
2007
Session ID : 2A1-O06
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2A1-O06 On-chip cell manipulation systems : Part4 : On-demand supply of fluorescence reagent
Fumihito AraiNaoki InomataYoko YamanishiRyuto OokawaraYu-Ching Lin
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Abstract
We demonstrate visualization method of carbon nanotubes (CNTs) in water with fluorescent microscopy through quenching effect that cause a decrease of fluorescent intensity around CNTs. Reversal contrast in fluorescence imaging of the CNTs was observed in fluorescent dye solution. The novel on-demand and on-chip control system of concentration of fluorescent dye has been developed in order to adjust appropriate concentration to observe quenching effect of CNTs. We could observe CNTs under a constant excitation light for more than ten minutes. Dielectrophoretic force was used for CNTs deposition onto electrodes that consist of transparent conductive film. The observation using a scanning electron microscope revealed that CNTs deposited onto electrodes were observed with fluorescent microscopy. Using four gage method, electrical resgistance of CNTs were measured. It can be applied for biological sensing devises with nanomanipulation.
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© 2007 The Japan Society of Mechanical Engineers
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