Abstract
In this study, a temperature sensing and controlling microfluid chip has been developed for cell immobilization using a thermo-sensitive hydrogel (PNIPAAm). The ITO (Indium Tin Oxide) microheaters and thermosensors, fabricated by micromachining technology, perform in situ fluid heating and temperature sensing. The PDMS-based micromagnetic stirrers make microscale fluid mixing to provide the temperature stability in the microchannel. All temperature sensing and controlling devices are integrated on a chip, in which yeast cell immobilization is performed by the gelation of the PNIPAAm solution.
