In tissue engineering field, many engineered approaches is researched, including artificial heart, artificial joint and synthetic vascular prosthesis. We have been developing scaffolds of three-dimensional synthetic vascular prosthesis in tailonnade. Vascular endothelial cells (FILIVEC) attached on inner surface of the scaffold has effects as anticoagulant action. Structures of the inner surface are important to adhesion of cells. In the present study, we aim to quantify inner surface condition of the scaffold by observing behavior and morphology of HIJVEC. For that purpose, we recreated inner surface of the scaffold on PDMS (polydimethilsiloxane) sheet by micro fabrication. As a result, we measured the resist semiround pattern which had 8 μm diameter and 5 μm bight, and looked the concave pattern on PDMS. And, HUVEC adhered on patterned PDMS. In addition, we fabricated arterioles model by photolithography. We succeeded at making arteriole tube model which had 1.1 cm length, 300〜400 μm diameter.