Abstract
We succeeded in making a long time observation system of individual cells using PDMS microchip which has a cage made of permeable membrane. The cage is made by photolithography and it has fine resolution. The height of the cage is about 2 μm. Optical tweezers is used to manipulate each individual cell. We transported cells into the cage using optical tweezers and changed solution around cells. The size change of each cell was monitored for long time stably. This system provides us a stable environment to observe cells in various conditions and it is useful to investigate unknown biological mechanisms of cells.
