2A1-J02 線虫の追跡を実現する蛍光顕微鏡システム

抄録

Many neuroscientists use C. elegans as a model organism to investigate neural functions. In order to evaluate neural activities in C. elegans, it is necessary to monitor Ca2+ concentration in neurons with a fluorescent microscope. However, it is difficult to observe C. elegans for a long time at a high magnification because of its movement. To solve this problem, we developed a microscope system which can track C. elegans and observe fluorescence emitted from fluorescent materials in C. elegans. As a result, we could continue to observe fluorescence emitted from GET introduced in a pharynx site of moving C.elegans for 30 seconds at 40x magnification.