2A1-J13 Rotation Speed Manipulation of Na^+-driven Flagellum by Local Spout from Nanopipette

Abstract

Local environmental control technique is desired to analyze the properties of a single cell. In this paper, local environmental control by nanopipette is applied to the rotation speed manipulation of bacterial flagellum which is a rotary molecular machine. We demonstrate dynamic and iterative rotation speed manipulation of Na^+-driven flagellum by switching of the local spout between Na^+-containing and Na^+-free solution with dual pipette. It is shown that the rotation speed might be controllable by changing the spouting volume of Na^+-containing solution with multiplying the DC voltage.