抄録
We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and they are fused by an electrical field. For the current study, the microfluidic chip was designed to have 3 parts for this process. Then, all-in-one unified microfluidic chip was successfully fabricated. Basic functions of these parts are verified independently.
