1A2-B03 Local Injection of Nanoprobe into C. elegans using Nanomanipulation system inside Environmental-SEM(Nano/Micro Manipulation System)

Abstract

In this paper, a novel local injection system was proposed using a fluorescent coated nanoprobe into Caenorhabditis elegans (C. elegans) using nanomanipulation system inside electron microscope (E-SEM). The nanoprobe was fabricated by focused ion beam (FIB) process and coated by a fluorescent dye. Its size is needed to be small for a minimal damage with a sharp tip and enough rigidity for the nanoinjection. The proposed direct nanoinjection technique is important to transport fluorescent materials or specific biological proteins into its specific cell for In-vivo experiment.