Abstract
For the present study, we propose a high frequency on-chip electric knife to fabricate micro-object. High current and voltage input was applied to the edge of electric knife of the order of sub-micron. The local ablation was carried by discharging micro-plasma from the edge of the electric knife. The most of the part of the knife was insulated except a limited region where is controlled by the photolithography technique. This prevents from generating bubbles from micro-electrode when the voltage is applied. We have confirmed the partial ablation of the cell as well as a discharge of plasma in the limited region. Expansion of the current proposed research will contribute to the cell fabrication for to the field of gene engineering and developmental engineering.