Abstract
In this paper, we proposed cell investigation tool for measurement of physiological conditions of single cell. We also developed new temperature measurement method using temperature dependence of the indicator. The cell investigation tool encapsulates indicators. These tools can be manipulated in a solution by optical tweezers. First, we classified cell investigation tools in four categories. Then, we proposed image analysis of the tools using the color information for temperature measurement. We calibrated the temperature using the tool impregnated with quantum dot by several color information such as HSV, YCrCb. Y and Cr represent the monotone decrease according to the temperature increase, while H and Cb do not represent monotone variation. Measurement value by Cr is stable than the values by Y and V information because the color difference is robust against brightness fluctuation. Therefore, we used Cr information for temperature measurement. Sensitivity is -1.3 %/K and accuracy is 0.3 K.
