Abstract
We've been working for developing scaffold free cell delivery system, and show good regeneration of rabbit osteochondral defect until more than three years by implantation of molded mesenchymal stem cells construct without use of exogenous factors. The entire procedure is simple, yet, it takes time and labor to build a single construct by even experienced hands. Therefore, this study was aimed to develop a cell processing robot for building Scaffold free HD MACs (High-density mesenchymal stem cell autologous constructs) for cartilage regeneration. In result, the scaffold free HD MACs could be obtained as same as manual procedure without contamination. We also implanted the construct into rabbit osteochondral defect in the knee. In this study, we developed the cell processing robot for cartilage regeneration. The entire system is almost GMP ready. We are preparing clinical trials in Kyushu university hospital for HD MACs implantation to cartilage defect with this robot system.
