This study aims to define a novel evaluation method of the settings of a road racer. To make an evaluation criteria of the setting of saddle height, the authors focused on lower muscular activities during cycling exercise and have thought an estimation method using surface electromyography and statistical processing. In our previous work, the experimental result indicated a small difference between the system output and the subjective aspect of an expert cyclist. Then, this paper reconsider the data processing method with reconstructing the experimental device. A symmetrical boundary condition is adopted in the process of First Fourier Transformation of electromyography. As the result, the effect of muscular fatigue clearly appeared in frequency analysis, so that the saddle height continuously dropped. This paper describe the technical materials and discusses the experimental results.