抄録
In this study, we present a smooth enucleation process on a microfluidic chip. To improve the success rate of enucleation and increasing the potential viability of the enucleated oocyte, a microfluidic system is specially designed. Magnetically driven MicroTool (MMT) driven with ultrasonic vibration control the flow distribution in the microchammber. Designed microchannel with height differences are for the purpose of confining oocyte with its nucleus located in the withdrawal microchannel to achieve the high precision control in cutting volume. By utilizing these aspects, first oocytes are sequentially loaded to the operation port; MMT constrains the oocyte location and controls the distribution of micro flow simultaneously letting hydraulic force cut off the nucleus from the oocyte. Enucleated oocyte is smooth in shape and the incision is closed. With cutting volume control, this system can achieve high-speed enucleation with less damage while removing the nucleus from an oocyte in a small volume. Since the flow distribution could be managed by MMT, therefore, the new system is proposed here to show advantages by the means of speed, cutting precision and great potentiality on continuous cutting process.
